Interaction Details


Details of experiments between Q96NT1 and Q16760
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: NAP1L5
Fragment: 1-182
Construct: endogenous protein (Jurkat)

Protein B

Protein: DGKD
Fragment: 1205-1214
Site: PBM
Sequence: LSRSAPAVEA
Construct: Biotin-ttds-LSRSAPAVEA
Average holdup BI: -0.35
Immobilized partner concentration (10-6M): 10
P value: 3.19
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 37942667
Experimental Details

-log10(P Two-sided unpaired T-test (3 vs 6))