Interaction Details


Details of experiments between Q96L92 and Q9HB71
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: avi(biotin)-his6-MBP-TEVsite-PDZ

Protein B

Protein: CACYBP
Fragment: 1-228
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.93
Immobilized partner concentration (10-6M): 10
P value: 14.49
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-27
Number of measurements: 6
Measured pKd: 6.16
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 6))

Experiment series 2

Protein A

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: avi(biotin)-his6-MBP-TEVsite-PDZ

Protein B

Protein: CACYBP
Fragment: 1-228
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.9
Immobilized partner concentration (10-6M): 10
P value: 15.75
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-08-03
Number of measurements: 3
Measured pKd: 5.96
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 3

Protein A

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: avi(biotin)-his6-MBP-TEVsite-PDZ

Protein B

Protein: CACYBP
Fragment: 1-228
Construct: endogenous protein (SH-SY5Y)
Average holdup BI: 0.9
Immobilized partner concentration (10-6M): 10
P value: 12.28
Experiment method: nHU_SH-SY5Y
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-27
Number of measurements: 6
Measured pKd: 5.94
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 6))

Experiment series 4

Protein A

Protein: SNX27
Fragment: 1-541
Construct: avi(biotin)-his6-MBP-TEVsite-SNX27

Protein B

Protein: CACYBP
Fragment: 1-228
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.72
Immobilized partner concentration (10-6M): 10
P value: 10.72
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-27
Number of measurements: 6
Measured pKd: 5.41
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 6))

Experiment series 5

Protein A

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: CACYBP
Fragment: 219-228
Site: PBM
Sequence: EKQAKGDTEF
Construct: Biotin-Ahx-EKQAKGDTEF
Average holdup BI: 0.07
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0.08
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 3.68
PUBMED ID: 36115835