Interaction Details


Details of experiments between Q96L92 and Q8N743
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: KIR3DL3
Fragment: 401-410
Site: PBM
Sequence: PKTPPTDTSV
Construct: biotin-ttds-PKTPPTDTSV
Average holdup BI: 0.16
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.32
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: KIR3DL3
Fragment: 401-410
Site: PBM
Sequence: PKTPPTDTSV
Construct: biotin-ttds-PKTPPTDTSV
Average holdup BI: 0.24
Holdup BI standard deviation: 0.03
Normalized holdup BI: 0.24
Normalized holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 3
Measured pKd: 4.28
PUBMED ID: 36115835