Interaction Details


Details of experiments between Q96L92 and Q6ZMN7
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PDZRN4
Fragment: 1027-1036
Site: PBM
Sequence: HNAFLSVTTV
Construct: Biotin-Ahx-HNAFLSVTTV
Average holdup BI: 0.07
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.07
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 3.65
PUBMED ID: 36115835
Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.