Interaction Details


Details of experiments between Q96L92 and Q68EM7
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SNX27
Fragment: 1-541
Construct: avi(biotin)-his6-MBP-TEVsite-SNX27

Protein B

Protein: ARHGAP17
Fragment: 1-881
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.77
Immobilized partner concentration (10-6M): 10
P value: 4.77
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-27
Number of measurements: 6
Measured pKd: 5.52
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 6))

Experiment series 2

Protein A

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: avi(biotin)-his6-MBP-TEVsite-PDZ

Protein B

Protein: ARHGAP17
Fragment: 1-881
Construct: endogenous protein (SH-SY5Y)
Average holdup BI: 0.72
Immobilized partner concentration (10-6M): 10
P value: 5.29
Experiment method: nHU_SH-SY5Y
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-27
Number of measurements: 6
Measured pKd: 5.41
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 6))

Experiment series 3

Protein A

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: avi(biotin)-his6-MBP-TEVsite-PDZ

Protein B

Protein: ARHGAP17
Fragment: 1-881
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.65
Immobilized partner concentration (10-6M): 10
P value: 4.43
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-27
Number of measurements: 6
Measured pKd: 5.27
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 6))

Experiment series 4

Protein A

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: avi(biotin)-his6-MBP-TEVsite-PDZ

Protein B

Protein: ARHGAP17
Fragment: 1-881
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.49
Immobilized partner concentration (10-6M): 10
P value: 5.91
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-08-03
Number of measurements: 3
Measured pKd: 4.99
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 5

Protein A

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ARHGAP17
Fragment: 872-881
Site: PBM
Sequence: IDNDTESTAL
Construct: biotin-ttds-IDNDTESTAL
Average holdup BI: 0.59
Normalized holdup BI: 0.59
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.97
PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: SNX27
Fragment: 1-541
Construct: endogenous protein (Jurkat)

Protein B

Protein: ARHGAP17
Fragment: 872-881
Site: PBM
Sequence: IDNDTESTAL
Construct: biotin-ttds-IDNDTESTAL
Experiment method: nHU-WB_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-08-03
Number of measurements: 3
Measured pKd: 4.93
PUBMED ID: 36542723