Interaction Details


Details of experiments between Q96L92 and Q15758
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: avi(biotin)-his6-MBP-TEVsite-PDZ

Protein B

Protein: SLC1A5
Fragment: 1-541
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.83
Immobilized partner concentration (10-6M): 10
P value: 4.62
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-27
Number of measurements: 6
Measured pKd: 5.7
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 6))

Experiment series 2

Protein A

Protein: SNX27
Fragment: 1-541
Construct: avi(biotin)-MBP-SNX27-his6

Protein B

Protein: SLC1A5
Fragment: 1-541
Construct: endogenous protein (Jurkat)
Experiment method: nHU-WB_Jurkat
Details of affinity fitting: hyperbolic binding equation (8 point titration)
Date of measurement: 2022-08-03
Number of measurements: 3
Measured pKd: 5.5
PUBMED ID: 36542723

Experiment series 3

Protein A

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SLC1A5
Fragment: 532-541
Site: PBM
Sequence: TVASEKESVM
Construct: biotin-ttds-TVASEKESVM
Average holdup BI: 0.75
Normalized holdup BI: 0.75
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.29
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: SNX27
Fragment: 1-541
Construct: avi(biotin)-his6-MBP-TEVsite-SNX27

Protein B

Protein: SLC1A5
Fragment: 1-541
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.59
Immobilized partner concentration (10-6M): 10
P value: 8.65
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-27
Number of measurements: 6
Measured pKd: 5.15
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 6))

Experiment series 5

Protein A

Protein: SNX27
Fragment: 1-541
Construct: endogenous protein (Jurkat)

Protein B

Protein: SLC1A5
Fragment: 532-541
Site: PBM
Sequence: TVASEKESVM
Construct: biotin-ttds-TVASEKESVM
Experiment method: nHU-WB_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-08-03
Number of measurements: 3
Measured pKd: 5.05
PUBMED ID: 36542723

Experiment series 6

Protein A

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: avi(biotin)-his6-MBP-TEVsite-PDZ

Protein B

Protein: SLC1A5
Fragment: 1-541
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.35
Immobilized partner concentration (10-6M): 10
P value: 4.04
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-08-03
Number of measurements: 3
Measured pKd: 4.74
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))