Interaction Details


Details of experiments between Q96L92 and O14578
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: avi(biotin)-his6-MBP-TEVsite-PDZ

Protein B

Protein: CIT
Fragment: 1-2027
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.83
Immobilized partner concentration (10-6M): 10
P value: 7.52
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-08-03
Number of measurements: 3
Measured pKd: 5.7
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 2

Protein A

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: avi(biotin)-his6-MBP-TEVsite-PDZ

Protein B

Protein: CIT
Fragment: 1-2027
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.76
Immobilized partner concentration (10-6M): 10
P value: 4.04
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-27
Number of measurements: 6
Measured pKd: 5.49
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 6))

Experiment series 3

Protein A

Protein: SNX27
Fragment: 1-541
Construct: endogenous protein (Jurkat)

Protein B

Protein: CIT
Fragment: 2018-2027
Site: PBM
Sequence: VNKVWDQSSV
Construct: biotin-ttds-VNKVWDQSSV
Experiment method: nHU-WB_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-08-03
Number of measurements: 3
Measured pKd: 5.18
PUBMED ID: 36542723

Experiment series 4

Protein A

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: CIT
Fragment: 2018-2027
Site: PBM
Sequence: VNKVWDQSSV
Construct: biotin-ttds-VNKVWDQSSV
Average holdup BI: 0.66
Normalized holdup BI: 0.66
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.11
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: SNX27
Fragment: 1-541
Construct: avi(biotin)-his6-MBP-TEVsite-SNX27

Protein B

Protein: CIT
Fragment: 1-2027
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.47
Immobilized partner concentration (10-6M): 10
P value: 1.48
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-27
Number of measurements: 6
The affinity was below the detection threshold of the assay.
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 6))