Interaction Details


Details of experiments between Q96L92 and A6NIM6
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SLC15A5
Fragment: 570-579
Site: PBM
Sequence: SSIDLWETAL
Construct: biotin-ttds-SSIDLWETAL
Average holdup BI: 0.71
Immobilized partner concentration (10-6M): 3.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 6.58
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SLC15A5
Fragment: 570-579
Site: PBM
Sequence: SSIDLWETAL
Construct: biotin-ttds-SSIDLWETAL
Average holdup BI: 0.89
Normalized holdup BI: 0.94
Immobilized partner concentration (10-6M): 11
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 6.32
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 3

Protein A

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SLC15A5
Fragment: 570-579
Site: PBM
Sequence: SSIDLWETAL
Construct: biotin-ttds-SSIDLWETAL
Average holdup BI: 0.8
Normalized holdup BI: 0.92
Immobilized partner concentration (10-6M): 11
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 6.19
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 4

Protein A

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SLC15A5
Fragment: 570-579
Site: PBM
Sequence: SSIDLWETAL
Construct: biotin-ttds-SSIDLWETAL
Average holdup BI: 0.86
Holdup BI standard deviation: 0.09
Normalized holdup BI: 0.86
Normalized holdup BI standard deviation: 0.09
Immobilized partner concentration (10-6M): 11
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 4
Measured pKd: 5.92
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SLC15A5
Fragment: 570-579
Site: PBM
Sequence: SSIDLWETAL
Construct: Biotin-ado-ado-SSIDLWETAL
Average holdup BI: 0.88
Normalized holdup BI: 0.88
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.72
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6