Interaction Details


Details of experiments between Q96KB5 and Q15700
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PBK
Fragment: 313-322
Site: PBM
Sequence: HIVEALETDV
Construct: biotin-ttds-HIVEALETDV

Protein B

Protein: DLG2
Fragment: 189-283
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.63
Normalized holdup BI: 0.63
Immobilized partner concentration (10-6M): 11.6
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.28
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: PBK
Fragment: 313-322
Site: PBM
Sequence: HIVEALETDV
Construct: biotin-ttds-HIVEALETDV

Protein B

Protein: DLG2
Fragment: 189-283
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.26
Immobilized partner concentration (10-6M): 5.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.9
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: PBK
Fragment: 313-322
Site: PBM
Sequence: HIVEALETDV
Construct: biotin-ttds-HIVEALETDV

Protein B

Protein: DLG2
Fragment: 93-186
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.21
Immobilized partner concentration (10-6M): 5.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.74
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: PBK
Fragment: 313-322
Site: PBM
Sequence: HIVEALETDV
Construct: biotin-ttds-HIVEALETDV

Protein B

Protein: DLG2
Fragment: 418-513
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.05
Normalized holdup BI: 0.05
Immobilized partner concentration (10-6M): 11.6
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.7
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: PBK
Fragment: 313-322
Site: PBM
Sequence: HIVEALETDV
Construct: biotin-ttds-HIVEALETDV

Protein B

Protein: DLG2
Fragment: 418-513
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 5.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835