Interaction Details

Details of experiments between Q96JY6 and Q15700
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: PDLIM2
Fragment: 343-352
Site: PBM
Sequence: SAPATLSSRA
Construct: Biotin-Ahx-SAPATLSSRA

Protein B

Protein: DLG2
Fragment: 189-283
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.03

Normalized holdup BI: 0.03

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 2