Interaction Details


Details of experiments between Q96JH8 and O14924
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: RADIL
Fragment: 973-1075
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RGS12
Fragment: 1438-1447
Site: PBM
Sequence: KTSAHHATFV
Construct: Biotin-ado-ado-KTSAHHATFV
Average holdup BI: 0.78
Normalized holdup BI: 0.78
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.38
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 2

Protein A

Protein: RADIL
Fragment: 973-1075
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RGS12
Fragment: 1438-1447
Site: PBM
Sequence: KTSAHHATFV
Construct: Biotin-Ahx-KTSAHHATFV
Average holdup BI: 0.74
Normalized holdup BI: 0.74
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.28
PUBMED ID: 36115835