Interaction Details


Details of experiments between Q96JB8 and Q92793
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MPP4
Fragment: 142-241
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: CBP
Fragment: 2433-2442
Site: PBM
Sequence: DTLEKFVEGL
Construct: Biotin-ttds-TGDTLEKFVEGL
Average holdup BI: 0.14
Immobilized partner concentration (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
Measured pKd: 3.95
PUBMED ID: 36115835