Interaction Details


Details of experiments between Q96J02 and NC_000001.11
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ITCH
Fragment: 1-903
Construct: endogenous protein (K562)

Protein B

Protein: 4945_HAP
Fragment: 203681644-203681669
Construct: Biotin-TCAGCCCTCCGTTTTGTCACCTACAC
Average holdup BI: -0.03
Immobilized partner concentration (10-6M): 5
P value: 0.11
Experiment method: nHU_K562
Details of affinity fitting: hyperbolic binding equation
Number of measurements: 3
The affinity was below the detection threshold of the assay.
Experimental Details

-log10(P Two-sided unpaired T-test (3 vs 6))

Experiment series 2

Protein A

Protein: ITCH
Fragment: 1-903
Construct: endogenous protein (K562)

Protein B

Protein: 51_HAP
Fragment: 203681832-203681862
Construct: Biotin-GAATTGAGAGGTATCTTACCGCTCCCACTCC
Average holdup BI: -0.13
Immobilized partner concentration (10-6M): 5
P value: 0.54
Experiment method: nHU_K562
Details of affinity fitting: hyperbolic binding equation
Number of measurements: 3
The affinity was below the detection threshold of the assay.
Experimental Details

-log10(P Two-sided unpaired T-test (3 vs 6))

Experiment series 3

Protein A

Protein: ITCH
Fragment: 1-903
Construct: endogenous protein (K562)

Protein B

Protein: 4945_WT
Fragment: 203681644-203681669
Modification: rs10751449/rs10736845
Construct: Biotin-TCAGCCCTCCGTATCGTCACCTACAC
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 5
P value: 0.12
Experiment method: nHU_K562
Details of affinity fitting: hyperbolic binding equation
Number of measurements: 3
The affinity was below the detection threshold of the assay.
Experimental Details

-log10(P Two-sided unpaired T-test (3 vs 6))

Experiment series 4

Protein A

Protein: ITCH
Fragment: 1-903
Construct: endogenous protein (K562)

Protein B

Protein: 51_WT
Fragment: 203681832-203681862
Modification: rs10751451
Construct: Biotin-GAATTGAGAGGTATCTTATCGCTCCCACTCC
Average holdup BI: -0.08
Immobilized partner concentration (10-6M): 5
P value: 0.33
Experiment method: nHU_K562
Details of affinity fitting: hyperbolic binding equation
Number of measurements: 3
The affinity was below the detection threshold of the assay.
Experimental Details

-log10(P Two-sided unpaired T-test (3 vs 6))