Interaction Details

Details of experiments between Q96IG2 and Q16760
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: FBXL20
Fragment: 1-436
Construct: endogenous protein (Jurkat)

Protein B

Protein: DGKD
Fragment: 1205-1214
Site: PBM
Sequence: LSRSAPAVEA
Construct: Biotin-ttds-LSRSAPAVEA

Average holdup BI: -0.24

Immobilized partner concentration (10^-6M): 10

P value: 0.44

Experiment method: nHU_Jurkat

Details of affinity fitting: hyperbolic binding equation

Number of measurements: 3

The affinity was below the detection threshold of the assay.

PUBMED ID: 37942667

Experimental Details

-log10(P Two-sided unpaired T-test (3 vs 6))