Interaction Details


Details of experiments between Q96GV9 and Q04917
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MACIR
Fragment: 162-171
Site: 14-3-3_pSer167
Modification: (SEP)167
Sequence: RAHSKSLDYL
Construct: RAHSK(pSer)LDYL (crude synthetic peptide)

Protein B

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta
Average holdup BI: 0.6
Holdup BI standard deviation: 0.12
Immobilized partner concentration (10-6M): 130
P value: 4.49
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.07
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))