Interaction Details


Details of experiments between Q96GV9 and P61981
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MACIR
Fragment: 127-136
Site: 14-3-3_pSer132
Modification: (SEP)132
Sequence: RRRMPSSGDK
Construct: RRRMP(pSer)SGDK (crude synthetic peptide)

Protein B

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma
Average holdup BI: 0.15
Holdup BI standard deviation: 0.2
Immobilized partner concentration (10-6M): 134
P value: 0.58
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 2

Protein A

Protein: MACIR
Fragment: 162-171
Site: 14-3-3_pSer167
Modification: (SEP)167
Sequence: RAHSKSLDYL
Construct: RAHSK(pSer)LDYL (crude synthetic peptide)

Protein B

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma
Average holdup BI: 0.59
Holdup BI standard deviation: 0.13
Immobilized partner concentration (10-6M): 134
P value: 3.95
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.03
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 3

Protein A

Protein: MACIR
Fragment: 162-171
Site: 14-3-3_pSer167
Modification: (SEP)167
Sequence: RAHSKSLDYL
Construct: RAHSK(pSer)LDYL (crude synthetic peptide)

Protein B

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma
Average holdup BI: 0.47
Holdup BI standard deviation: 0.18
Immobilized partner concentration (10-6M): 129
P value: 2.53
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.83
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 4

Protein A

Protein: MACIR
Fragment: 135-144
Site: 14-3-3_pSer140
Modification: (SEP)140
Sequence: DKCTKSLPYE
Construct: DKCTK(pSer)LPYE (crude synthetic peptide)

Protein B

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma
Average holdup BI: -0.31
Holdup BI standard deviation: 1.1
Immobilized partner concentration (10-6M): 115
P value: 0.25
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 5

Protein A

Protein: MACIR
Fragment: 162-171
Site: 14-3-3_pSer167
Modification: (SEP)167
Sequence: RAHSKSLDYL
Construct: RAHSK(pSer)LDYL (crude synthetic peptide)

Protein B

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma
Average holdup BI: 0.64
Holdup BI standard deviation: 0.09
Immobilized partner concentration (10-6M): 115
P value: 2.63
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.2
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))