Interaction Details


Details of experiments between Q96EV2 and P00519
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: RBM33
Fragment: 1-1170
Construct: endogenous protein (Jurkat)

Protein B

Protein: ABL1
Fragment: 56-121
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.92
Immobilized partner concentration (10-6M): 10
P value: 7.94
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
Measured pKd: 6.09
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 2

Protein A

Protein: RBM33
Fragment: 477-491
Site: PxxP_1
Sequence: LEQRSPPPPPPPPTL
Construct: Bio Ado Ado-LEQRSPPPPPPPPTL-CONH2

Protein B

Protein: ABL1
Fragment: 56-121
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 3

Protein A

Protein: RBM33
Fragment: 521-535
Site: PxxP_2
Sequence: VFPRERPVRPALQPP
Construct: Bio Ado Ado-VFPRERPVRPALQPP-CONH2

Protein B

Protein: ABL1
Fragment: 56-121
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 4

Protein A

Protein: RBM33
Fragment: 547-561
Site: PxxP_3
Sequence: GSATTRPFIPPRQPF
Construct: Bio Ado Ado-GSATTRPFIPPRQPF-CONH2

Protein B

Protein: ABL1
Fragment: 56-121
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 5

Protein A

Protein: RBM33
Fragment: 706-720
Site: PxxP_4
Sequence: SNLRELPIAPSHVIE
Construct: Bio Ado Ado-SNLRELPIAPSHVIE-CONH2

Protein B

Protein: ABL1
Fragment: 56-121
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 6

Protein A

Protein: RBM33
Fragment: 757-771
Site: PxxP_5
Sequence: TEVKVKPASPVAQPK
Construct: Bio Ado Ado-TEVKVKPASPVAQPK-CONH2

Protein B

Protein: ABL1
Fragment: 56-121
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 7

Protein A

Protein: RBM33
Fragment: 926-940
Site: PxxP_6
Sequence: PLHKVLPIKPADVEE
Construct: Bio Ado Ado-PLHKVLPIKPADVEE-CONH2

Protein B

Protein: ABL1
Fragment: 56-121
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680