Interaction Details


Details of experiments between Q96DX4 and P49418
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: RSPRY1
Fragment: 74-88
Site: PxxP_2
Sequence: RSQPRDPVRPPRRGR
Construct: Bio Ado Ado-RSQPRDPVRPPRRGR-CONH2

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.83
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 5.53
PUBMED ID: 38995680

Experiment series 2

Protein A

Protein: RSPRY1
Fragment: 1-576
Construct: endogenous protein (Jurkat)

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.6
Immobilized partner concentration (10-6M): 10
P value: 3.31
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
Measured pKd: 5.18
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 3

Protein A

Protein: RSPRY1
Fragment: 71-85
Site: PxxP_1
Sequence: ADTRSQPRDPVRPPR
Construct: Bio Ado Ado-ADTRSQPRDPVRPPR-CONH2

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.66
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 2
Measured pKd: 5.09
PUBMED ID: 38995680

Experiment series 4

Protein A

Protein: RSPRY1
Fragment: 84-98
Site: PxxP_3
Sequence: PRRGRGPHEPRRKKQ
Construct: Bio Ado Ado-PRRGRGPHEPRRKKQ-CONH2

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 3.49
PUBMED ID: 38995680