Interaction Details


Details of experiments between Q96DR5 and Q96QZ7
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: BPIFA2
Fragment: 240-249
Site: PBM
Sequence: QHKTQLQTLI
Construct: biotin-ado-ado-QHKTQLQTLI

Protein B

Protein: MAGI1
Fragment: 456-580
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.23
Normalized holdup BI: 0.26
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.32
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 2

Protein A

Protein: BPIFA2
Fragment: 240-249
Site: PBM
Sequence: QHKTQLQTLI
Construct: biotin-ado-ado-QHKTQLQTLI

Protein B

Protein: MAGI1
Fragment: 1-106
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.07
Normalized holdup BI: 0.07
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.66
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.