Interaction Details


Details of experiments between Q96BN8 and Q9P202
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: OTULIN
Fragment: 343-352
Site: PBM
Sequence: PVRVCEETSL
Construct: Biotin-ado-ado-PVRVCEETSL

Protein B

Protein: DFNB31
Fragment: 136-226
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.51
Normalized holdup BI: 0.51
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.81
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 2

Protein A

Protein: OTULIN
Fragment: 343-352
Site: PBM
Sequence: PVRVCEETSL
Construct: biotin-ttds-PVRVCEETSL

Protein B

Protein: DFNB31
Fragment: 136-226
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.31
Normalized holdup BI: 0.31
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.42
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: OTULIN
Fragment: 343-352
Site: PBM
Sequence: PVRVCEETSL
Construct: biotin-ttds-PVRVCEETSL

Protein B

Protein: DFNB31
Fragment: 263-379
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.06
Normalized holdup BI: 0.06
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.58
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: OTULIN
Fragment: 343-352
Site: PBM
Sequence: PVRVCEETSL
Construct: biotin-ttds-PVRVCEETSL

Protein B

Protein: DFNB31
Fragment: 813-904
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.05
Normalized holdup BI: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.49
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: OTULIN
Fragment: 343-352
Site: PBM
Sequence: PVRVCEETSL
Construct: Biotin-ado-ado-PVRVCEETSL

Protein B

Protein: DFNB31
Fragment: 263-379
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.01
Normalized holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 6

Protein A

Protein: OTULIN
Fragment: 343-352
Site: PBM
Sequence: PVRVCEETSL
Construct: Biotin-ado-ado-PVRVCEETSL

Protein B

Protein: DFNB31
Fragment: 813-904
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.02
Normalized holdup BI: -0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6