Interaction Details


Details of experiments between Q92796 and Q8IWK6
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: DLG3
Fragment: 221-314
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ADGRA3
Fragment: 1312-1321
Site: PBM
Sequence: TGLWKHETTV
Construct: biotin-ado-ado-TGLWKHETTV
Average holdup BI: 0.69
Normalized holdup BI: 0.73
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.25
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 2

Protein A

Protein: DLG3
Fragment: 381-476
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ADGRA3
Fragment: 1312-1321
Site: PBM
Sequence: TGLWKHETTV
Construct: biotin-ado-ado-TGLWKHETTV
Average holdup BI: 0.42
Normalized holdup BI: 0.49
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.77
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 3

Protein A

Protein: DLG3
Fragment: 124-219
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ADGRA3
Fragment: 1312-1321
Site: PBM
Sequence: TGLWKHETTV
Construct: biotin-ado-ado-TGLWKHETTV
Average holdup BI: 0.42
Normalized holdup BI: 0.44
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.69
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.