Interaction Details


Details of experiments between Q92796 and Q7Z628
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: DLG3
Fragment: 221-314
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: NET1
Fragment: 587-596
Site: PBM
Sequence: SGGKRKETLV
Construct: biotin-ttds-SGGKRKETLV
Average holdup BI: 0.93
Immobilized partner concentration (10-6M): 35
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
Measured pKd: 5.63
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: DLG3
Fragment: 221-314
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: NET1
Fragment: 587-596
Site: PBM
Sequence: SGGKRKETLV
Construct: biotin-ttds-SGGKRKETLV
Average holdup BI: 0.64
Holdup BI standard deviation: 0
Normalized holdup BI: 0.8
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 17.9
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 5.43
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: DLG3
Fragment: 221-314
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: NET1
Fragment: 587-596
Site: PBM
Sequence: SGGKRKETLV
Construct: Biotin-ado-ado-SGGKRKETLV
Average holdup BI: 0.74
Normalized holdup BI: 0.78
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.38
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 4

Protein A

Protein: DLG3
Fragment: 221-314
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: NET1
Fragment: 587-596
Site: PBM
Sequence: SGGKRKETLV
Construct: biotin-ttds-SGGKRKETLV
Average holdup BI: 0.61
Normalized holdup BI: 0.73
Immobilized partner concentration (10-6M): 17.9
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.26
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 5

Protein A

Protein: DLG3
Fragment: 381-476
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: NET1
Fragment: 587-596
Site: PBM
Sequence: SGGKRKETLV
Construct: Biotin-ado-ado-SGGKRKETLV
Average holdup BI: 0.56
Normalized holdup BI: 0.66
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.11
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 6

Protein A

Protein: DLG3
Fragment: 381-476
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: NET1
Fragment: 587-596
Site: PBM
Sequence: SGGKRKETLV
Construct: biotin-ttds-SGGKRKETLV
Average holdup BI: 0.5
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.63
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 17.9
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 5.05
PUBMED ID: 36115835

Experiment series 7

Protein A

Protein: DLG3
Fragment: 381-476
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: NET1
Fragment: 587-596
Site: PBM
Sequence: SGGKRKETLV
Construct: biotin-ttds-SGGKRKETLV
Average holdup BI: 0.48
Normalized holdup BI: 0.58
Immobilized partner concentration (10-6M): 17.9
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.95
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 8

Protein A

Protein: DLG3
Fragment: 381-476
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: NET1
Fragment: 587-596
Site: PBM
Sequence: SGGKRKETLV
Construct: biotin-ttds-SGGKRKETLV
Average holdup BI: 0.65
Immobilized partner concentration (10-6M): 35
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
Measured pKd: 4.76
PUBMED ID: 36115835

Experiment series 9

Protein A

Protein: DLG3
Fragment: 124-219
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: NET1
Fragment: 587-596
Site: PBM
Sequence: SGGKRKETLV
Construct: Biotin-ado-ado-SGGKRKETLV
Average holdup BI: 0.46
Normalized holdup BI: 0.48
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.76
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 10

Protein A

Protein: DLG3
Fragment: 124-219
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: NET1
Fragment: 587-596
Site: PBM
Sequence: SGGKRKETLV
Construct: biotin-ttds-SGGKRKETLV
Average holdup BI: 0.39
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.4
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 17.9
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.61
PUBMED ID: 36115835

Experiment series 11

Protein A

Protein: DLG3
Fragment: 124-219
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: NET1
Fragment: 587-596
Site: PBM
Sequence: SGGKRKETLV
Construct: biotin-ttds-SGGKRKETLV
Average holdup BI: 0.56
Immobilized partner concentration (10-6M): 35
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
Measured pKd: 4.59
PUBMED ID: 36115835

Experiment series 12

Protein A

Protein: DLG3
Fragment: 124-219
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: NET1
Fragment: 587-596
Site: PBM
Sequence: SGGKRKETLV
Construct: biotin-ttds-SGGKRKETLV
Average holdup BI: 0.29
Normalized holdup BI: 0.29
Immobilized partner concentration (10-6M): 17.9
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.4
Experimental Details

not reported internal standards of holdup layout #5