Interaction Details


Details of experiments between Q92796 and Q6ZTQ3
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: DLG3
Fragment: 221-314
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RASSF6
Fragment: 360-369
Site: PBM
Sequence: KLVIKTETTV
Construct: biotin-ttds-KLVIKTETTV
Average holdup BI: 0.69
Normalized holdup BI: 0.86
Immobilized partner concentration (10-6M): 20.7
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.53
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: DLG3
Fragment: 221-314
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RASSF6
Fragment: 360-369
Site: PBM
Sequence: KLVIKTETTV
Construct: biotin-ttds-KLVIKTETTV
Average holdup BI: 0.45
Immobilized partner concentration (10-6M): 9
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 5.06
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: DLG3
Fragment: 124-219
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RASSF6
Fragment: 360-369
Site: PBM
Sequence: KLVIKTETTV
Construct: biotin-ttds-KLVIKTETTV
Average holdup BI: 0.38
Normalized holdup BI: 0.39
Immobilized partner concentration (10-6M): 20.7
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.52
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: DLG3
Fragment: 124-219
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RASSF6
Fragment: 360-369
Site: PBM
Sequence: KLVIKTETTV
Construct: biotin-ttds-KLVIKTETTV
Average holdup BI: 0.18
Immobilized partner concentration (10-6M): 9
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.43
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: DLG3
Fragment: 381-476
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RASSF6
Fragment: 360-369
Site: PBM
Sequence: KLVIKTETTV
Construct: biotin-ttds-KLVIKTETTV
Average holdup BI: 0.27
Normalized holdup BI: 0.34
Immobilized partner concentration (10-6M): 20.7
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.43
PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: DLG3
Fragment: 381-476
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RASSF6
Fragment: 360-369
Site: PBM
Sequence: KLVIKTETTV
Construct: biotin-ttds-KLVIKTETTV
Average holdup BI: 0.13
Immobilized partner concentration (10-6M): 9
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.25
PUBMED ID: 36115835