Interaction Details


Details of experiments between Q92796 and Q13574
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: DLG3
Fragment: 221-314
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: DGKZ
Fragment: 919-928
Site: PBM
Sequence: IQREDQETAV
Construct: biotin-ttds-IQREDQETAV
Average holdup BI: 0.38
Holdup BI standard deviation: 0
Normalized holdup BI: 0.47
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 13.5
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.88
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: DLG3
Fragment: 221-314
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: DGKZ
Fragment: 919-928
Site: PBM
Sequence: IQREDQETAV
Construct: biotin-ttds-IQREDQETAV
Average holdup BI: 0.48
Normalized holdup BI: 0.51
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.81
PUBMED ID: 36115835
Experimental Details

not reported internal standards of holdup layout #6

Experiment series 3

Protein A

Protein: DLG3
Fragment: 221-314
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: DGKZ
Fragment: 919-928
Site: PBM
Sequence: IQREDQETAV
Construct: biotin-ttds-IQREDQETAV
Average holdup BI: 0.24
Immobilized partner concentration (10-6M): 6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.8
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: DLG3
Fragment: 381-476
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: DGKZ
Fragment: 919-928
Site: PBM
Sequence: IQREDQETAV
Construct: biotin-ttds-IQREDQETAV
Average holdup BI: 0.2
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.25
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 13.5
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.42
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: DLG3
Fragment: 124-219
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: DGKZ
Fragment: 919-928
Site: PBM
Sequence: IQREDQETAV
Construct: biotin-ttds-IQREDQETAV
Average holdup BI: 0.11
Immobilized partner concentration (10-6M): 6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.33
PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: DLG3
Fragment: 124-219
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: DGKZ
Fragment: 919-928
Site: PBM
Sequence: IQREDQETAV
Construct: biotin-ttds-IQREDQETAV
Average holdup BI: 0.18
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.19
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 13.5
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.26
PUBMED ID: 36115835

Experiment series 7

Protein A

Protein: DLG3
Fragment: 124-219
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: DGKZ
Fragment: 919-928
Site: PBM
Sequence: IQREDQETAV
Construct: biotin-ttds-IQREDQETAV
Average holdup BI: 0.16
Normalized holdup BI: 0.17
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.08
PUBMED ID: 36115835
Experimental Details

not reported internal standards of holdup layout #6

Experiment series 8

Protein A

Protein: DLG3
Fragment: 381-476
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: DGKZ
Fragment: 919-928
Site: PBM
Sequence: IQREDQETAV
Construct: biotin-ttds-IQREDQETAV
Average holdup BI: 0.13
Normalized holdup BI: 0.15
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4
PUBMED ID: 36115835
Experimental Details

not reported internal standards of holdup layout #6

Experiment series 9

Protein A

Protein: DLG3
Fragment: 381-476
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: DGKZ
Fragment: 919-928
Site: PBM
Sequence: IQREDQETAV
Construct: biotin-ttds-IQREDQETAV
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835