Interaction Details


Details of experiments between Q92796 and P03409
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: DLG3
Fragment: 221-314
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HTLV1-TAX1
Fragment: 344-353
Site: PBM
Sequence: SEKHFRETEV
Construct: biotin-ttds-SEKHFRETEV
Average holdup BI: 0.65
Holdup BI standard deviation: 0
Normalized holdup BI: 0.81
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 15
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 5.56
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: DLG3
Fragment: 221-314
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HTLV1-TAX1
Fragment: 344-353
Site: PBM
Sequence: SEKHFRETEV
Construct: biotin-ttds-SEKHFRETEV
Average holdup BI: 0.83
Holdup BI standard deviation: 0.17
Immobilized partner concentration (10-6M): 19
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
Measured pKd: 5.49
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: DLG3
Fragment: 221-314
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HTLV1-TAX1
Fragment: 344-353
Site: PBM
Sequence: SEKHFRETEV
Construct: Biotin-ado-ado-SEKHFRETEV
Average holdup BI: 0.77
Normalized holdup BI: 0.81
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.45
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 4

Protein A

Protein: DLG3
Fragment: 381-476
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HTLV1-TAX1
Fragment: 344-353
Site: PBM
Sequence: SEKHFRETEV
Construct: biotin-ttds-SEKHFRETEV
Average holdup BI: 0.57
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0.73
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 15
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 5.34
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: DLG3
Fragment: 381-476
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HTLV1-TAX1
Fragment: 344-353
Site: PBM
Sequence: SEKHFRETEV
Construct: Biotin-ado-ado-SEKHFRETEV
Average holdup BI: 0.61
Normalized holdup BI: 0.72
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.23
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 6

Protein A

Protein: DLG3
Fragment: 381-476
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HTLV1-TAX1
Fragment: 344-353
Site: PBM
Sequence: SEKHFRETEV
Construct: biotin-ttds-SEKHFRETEV
Average holdup BI: 0.68
Holdup BI standard deviation: 0.08
Immobilized partner concentration (10-6M): 19
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
Measured pKd: 5.11
PUBMED ID: 36115835

Experiment series 7

Protein A

Protein: DLG3
Fragment: 124-219
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HTLV1-TAX1
Fragment: 344-353
Site: PBM
Sequence: SEKHFRETEV
Construct: Biotin-ado-ado-SEKHFRETEV
Average holdup BI: 0.39
Normalized holdup BI: 0.41
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.63
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 8

Protein A

Protein: DLG3
Fragment: 124-219
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HTLV1-TAX1
Fragment: 344-353
Site: PBM
Sequence: SEKHFRETEV
Construct: biotin-ttds-SEKHFRETEV
Average holdup BI: 0.33
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0.34
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 15
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.58
PUBMED ID: 36115835

Experiment series 9

Protein A

Protein: DLG3
Fragment: 124-219
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HTLV1-TAX1
Fragment: 344-353
Site: PBM
Sequence: SEKHFRETEV
Construct: biotin-ttds-SEKHFRETEV
Average holdup BI: 0.31
Immobilized partner concentration (10-6M): 19
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
Measured pKd: 4.41
PUBMED ID: 36115835