Interaction Details


Details of experiments between Q92793 and Q9UPQ7
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: CBP
Fragment: 2433-2442
Site: PBM
Sequence: DTLEKFVEGL
Construct: Biotin-ttds-TGDTLEKFVEGL

Protein B

Protein: PDZRN3
Fragment: 245-339
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: -0.01
Normalized holdup BI: -0.01
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
P value: 0
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: CBP
Fragment: 2433-2442
Site: PBM
Sequence: DTLEKFVEGL
Construct: Biotin-ttds-TGDTLEKFVEGL

Protein B

Protein: PDZRN3
Fragment: 245-339
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 0
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: 0
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 0
P value: 0
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835