Interaction Details


Details of experiments between Q92793 and Q9H8Y8
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: CBP
Fragment: 2433-2442
Site: PBM
Sequence: DTLEKFVEGL
Construct: Biotin-ttds-TGDTLEKFVEGL

Protein B

Protein: GORASP2
Fragment: 1-208
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.16
Immobilized partner concentration (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
Measured pKd: 4.03
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: CBP
Fragment: 2433-2442
Site: PBM
Sequence: DTLEKFVEGL
Construct: Biotin-ttds-TGDTLEKFVEGL

Protein B

Protein: GORASP2
Fragment: 1-208
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.01
Normalized holdup BI: -0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835