Interaction Details


Details of experiments between Q92793 and O96018
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: CBP
Fragment: 2433-2442
Site: PBM
Sequence: DTLEKFVEGL
Construct: Biotin-ttds-TGDTLEKFVEGL

Protein B

Protein: APBA3
Fragment: 482-569
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.14
Immobilized partner concentration (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
Measured pKd: 3.95
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: CBP
Fragment: 2433-2442
Site: PBM
Sequence: DTLEKFVEGL
Construct: Biotin-ttds-TGDTLEKFVEGL

Protein B

Protein: APBA3
Fragment: 390-479
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.13
Immobilized partner concentration (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
Measured pKd: 3.93
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: CBP
Fragment: 2433-2442
Site: PBM
Sequence: DTLEKFVEGL
Construct: Biotin-ttds-TGDTLEKFVEGL

Protein B

Protein: APBA3
Fragment: 390-479
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.01
Normalized holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835