Interaction Details


Details of experiments between Q92743 and Q9NQS3
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: HTRA1
Fragment: 365-480
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: NECTIN3
Fragment: 540-549
Site: PBM
Sequence: SVISRREWYV
Construct: biotin-ado-ado-SVISRREWYV
Average holdup BI: 0.69
Normalized holdup BI: 0.86
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.62
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.