Interaction Details

Details of experiments between Q8WXI2 and Q8TEW8
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: CNKSR2
Fragment: 1025-1034
Site: PBM
Sequence: HTHSYIETHV
Construct: Biotin-Ahx-HTHSYIETHV

Protein B

Protein: PARD3B
Fragment: 184-299
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0.04

Normalized holdup BI: -0.04

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835