Interaction Details


Details of experiments between Q8WXE0 and Q14155
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: CASKIN2
Fragment: 1-1202
Construct: endogenous protein (Jurkat)

Protein B

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.55
Immobilized partner concentration (10-6M): 10
P value: 3.28
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
Measured pKd: 5.09
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 2

Protein A

Protein: CASKIN2
Fragment: 862-876
Site: PxxP_5
Sequence: RARRKGPPPPPPKRL
Construct: Bio Ado Ado-RARRKGPPPPPPKRL-CONH2

Protein B

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.28
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.36
PUBMED ID: 38995680

Experiment series 3

Protein A

Protein: CASKIN2
Fragment: 863-877
Site: PxxP_6
Sequence: ARRKGPPPPPPKRLS
Construct: Bio Ado Ado-ARRKGPPPPPPKRLS-CONH2

Protein B

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.21
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 2
Measured pKd: 4.19
PUBMED ID: 38995680

Experiment series 4

Protein A

Protein: CASKIN2
Fragment: 864-878
Site: PxxP_7
Sequence: RRKGPPPPPPKRLSS
Construct: Bio Ado Ado-RRKGPPPPPPKRLSS-CONH2

Protein B

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.2
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.15
PUBMED ID: 38995680

Experiment series 5

Protein A

Protein: CASKIN2
Fragment: 1121-1135
Site: PxxP_1
Sequence: PRLGPRPVPPPRPES
Construct: Bio Ado Ado-PRLGPRPVPPPRPES-CONH2

Protein B

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.13
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 3.93
PUBMED ID: 38995680

Experiment series 6

Protein A

Protein: CASKIN2
Fragment: 785-799
Site: PxxP_4
Sequence: PATPPDPPRPKRRSH
Construct: Bio Ado Ado-PATPPDPPRPKRRSH-CONH2

Protein B

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.12
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 3.88
PUBMED ID: 38995680

Experiment series 7

Protein A

Protein: CASKIN2
Fragment: 683-697
Site: PxxP_2
Sequence: GGPEPLPLPPARSPS
Construct: Bio Ado Ado-GGPEPLPLPPARSPS-CONH2

Protein B

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.07
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 3.65
PUBMED ID: 38995680

Experiment series 8

Protein A

Protein: CASKIN2
Fragment: 782-796
Site: PxxP_3
Sequence: AGPPATPPDPPRPKR
Construct: Bio Ado Ado-AGPPATPPDPPRPKR-CONH2

Protein B

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.06
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 3.55
PUBMED ID: 38995680

Experiment series 9

Protein A

Protein: CASKIN2
Fragment: 960-974
Site: PxxP_8
Sequence: IKQRPKPAGPPPRET
Construct: Bio Ado Ado-IKQRPKPAGPPPRET-CONH2

Protein B

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 10

Protein A

Protein: CASKIN2
Fragment: 969-983
Site: PxxP_9
Sequence: PPPRETPVPPGLDFN
Construct: Bio Ado Ado-PPPRETPVPPGLDFN-CONH2

Protein B

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680