Interaction Details


Details of experiments between Q8WUF5 and Q9NZN5
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PPP1R13L
Fragment: 819-828
Site: PBM
Sequence: PRVKPQRSKV
Construct: biotin-ttds-PRVKPQRSKV

Protein B

Protein: ARHGEF12
Fragment: 67-151
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.05
Normalized holdup BI: 0.06
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.54
PUBMED ID: 36115835