Interaction Details


Details of experiments between Q8TF72 and Q96L92
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SHROOM3
Fragment: 1987-1996
Site: PBM
Sequence: GIFPTLTSPL
Construct: Biotin-Ahx-GIFPTLTSPL

Protein B

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.11
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0.12
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 3.91
PUBMED ID: 36115835