Interaction Details


Details of experiments between Q8TF72 and Q8TCX5
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SHROOM3
Fragment: 1987-1996
Site: PBM
Sequence: GIFPTLTSPL
Construct: Biotin-Ahx-GIFPTLTSPL

Protein B

Protein: RHPN1
Fragment: 504-602
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.24
Normalized holdup BI: 0.24
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.27
PUBMED ID: 36115835