Interaction Details


Details of experiments between Q8TF72 and O14907
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SHROOM3
Fragment: 1987-1996
Site: PBM
Sequence: GIFPTLTSPL
Construct: Biotin-Ahx-GIFPTLTSPL

Protein B

Protein: TX1BP3
Fragment: 7-124
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.07
Normalized holdup BI: 0.07
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.65
PUBMED ID: 36115835