Interaction Details


Details of experiments between Q8TF72 and A6NIM6
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SHROOM3
Fragment: 21-113
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SLC15A5
Fragment: 570-579
Site: PBM
Sequence: SSIDLWETAL
Construct: biotin-ttds-SSIDLWETAL
Average holdup BI: 0.16
Immobilized partner concentration (10-6M): 3.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.81
PUBMED ID: 36115835