Interaction Details

Details of experiments between Q8TF64 and P36507
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: GIPC3
Fragment: 107-202
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MAP2K2
Fragment: 391-400
Site: PBM
Sequence: QPGTPTRTAV
Construct: biotin-ttds-QPGTPTRTAV

Measured dissociation constant (10^-6M):

Standard deviation of dissociation constant (10^-6M): 0

Average holdup BI: 0.01

Normalized holdup BI: 0

Normalized holdup BI standard deviation: 0

Immobilized partner concentration (10^-6M): 10

P value: 0

Experiment method: SAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normal layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835