Interaction Details


Details of experiments between Q8TEW8 and Q9NYB5
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PARD3B
Fragment: 184-299
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SLCO1C1
Fragment: 703-712
Site: PBM
Sequence: NYWPGKETQL
Construct: Biotin-ado-ado-NYWPGKETQL
Average holdup BI: 0.12
Normalized holdup BI: 0.12
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.89
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 2

Protein A

Protein: PARD3B
Fragment: 184-299
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SLCO1C1
Fragment: 703-712
Site: PBM
Sequence: NYWPGKETQL
Construct: biotin-ttds-NYWPGKETQL
Average holdup BI: 0.04
Holdup BI standard deviation: 0.05
Normalized holdup BI: 0.05
Normalized holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 11.3
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 3
Measured pKd: 3.7
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: PARD3B
Fragment: 184-299
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SLCO1C1
Fragment: 703-712
Site: PBM
Sequence: NYWPGKETQL
Construct: biotin-ttds-NYWPGKETQL
Average holdup BI: 0
Immobilized partner concentration (10-6M): 5.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: PARD3B
Fragment: 184-299
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SLCO1C1
Fragment: 703-712
Site: PBM
Sequence: NYWPGKETQL
Construct: biotin-ttds-NYWPGKETQL
Average holdup BI: 0.02
Normalized holdup BI: 0.02
Immobilized partner concentration (10-6M): 11.3
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 5

Protein A

Protein: PARD3B
Fragment: 376-471
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SLCO1C1
Fragment: 703-712
Site: PBM
Sequence: NYWPGKETQL
Construct: biotin-ttds-NYWPGKETQL
Average holdup BI: -0.06
Immobilized partner concentration (10-6M): 5.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: PARD3B
Fragment: 490-594
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SLCO1C1
Fragment: 703-712
Site: PBM
Sequence: NYWPGKETQL
Construct: biotin-ttds-NYWPGKETQL
Average holdup BI: -0.08
Immobilized partner concentration (10-6M): 5.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835