Interaction Details


Details of experiments between Q8TEW8 and Q8N103
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PARD3B
Fragment: 184-299
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: TAGAP
Fragment: 722-731
Site: PBM
Sequence: QFQYAKESYI
Construct: biotin-ttds-QFQYAKESYI
Average holdup BI: 0.12
Holdup BI standard deviation: 0
Normalized holdup BI: 0.14
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 3.98
PUBMED ID: 36115835