Interaction Details


Details of experiments between Q8TEW8 and Q8IY63
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PARD3B
Fragment: 184-299
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: AMOTL1
Fragment: 947-956
Site: PBM
Sequence: PDGEMMEVLI
Construct: biotin-ado-ado-PDGEMMEVLI
Average holdup BI: 0.59
Normalized holdup BI: 0.59
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.95
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.