Interaction Details

Details of experiments between Q8TEW8 and P17386
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: PARD3B
Fragment: 184-299
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV31-E6
Fragment: 140-149
Site: PBM
Sequence: SRRPRTETQV
Construct: biotin-ttds-SRRPRTETQV

Average holdup BI: 0.01

Holdup BI standard deviation: 0.03

Normalized holdup BI: 0.02

Normalized holdup BI standard deviation: 0.03

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 4

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835