Interaction Details


Details of experiments between Q8TEW8 and O14745
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PARD3B
Fragment: 184-299
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: NHERF1
Fragment: 349-358
Site: PBM
Sequence: SKKNELFSNL
Construct: Biotin-Ahx-SKKNELFSNL
Average holdup BI: -0.02
Normalized holdup BI: -0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835