Interaction Details


Details of experiments between Q8TEW0 and Q9Q6P4
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PARD3
Fragment: 582-685
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: WNV-NS5
Fragment: 3424-3433
Site: PBM
Sequence: DTTLVEDTVL
Construct: biotin-(PEG)3-RYEDTTLVEDTVL
Average holdup BI: 0.36
Normalized holdup BI: 0.36
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.53
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: PARD3
Fragment: 582-685
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: WNV-NS5
Fragment: 3424-3433
Site: PBM
Sequence: DTTLVEDTVL
Construct: biotin-(PEG)3-RYEDTTLVEDTVL
Average holdup BI: 0.29
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 5
Measured pKd: 4.39
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: PARD3
Fragment: 264-372
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: WNV-NS5
Fragment: 3424-3433
Site: PBM
Sequence: DTTLVEDTVL
Construct: biotin-(PEG)3-RYEDTTLVEDTVL
Average holdup BI: 0.04
Holdup BI standard deviation: 0.1
Immobilized partner concentration (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 5
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: PARD3
Fragment: 454-550
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: WNV-NS5
Fragment: 3424-3433
Site: PBM
Sequence: DTTLVEDTVL
Construct: biotin-(PEG)3-RYEDTTLVEDTVL
Average holdup BI: 0.02
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 5
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835