Interaction Details


Details of experiments between Q8TEU7 and Q9BSE5
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: RAPGEF6
Fragment: 525-611
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: AGMAT
Fragment: 343-352
Site: PBM
Sequence: LCALPKVTTV
Construct: biotin-ttds-LCALPKVTTV
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: 0.07
Holdup BI standard deviation: 0
Normalized holdup BI: 0.07
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
P value: 0
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 3.63
PUBMED ID: 36115835