Interaction Details


Details of experiments between Q8TEU7 and Q92743
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: RAPGEF6
Fragment: 1592-1601
Site: PBM
Sequence: ADENEQVSAV
Construct: Biotin-Ahx-ADENEQVSAV

Protein B

Protein: HTRA1
Fragment: 365-480
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.1
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0.13
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 3.94
PUBMED ID: 36115835
Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.