Interaction Details


Details of experiments between Q8TEU7 and Q68DX3
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: RAPGEF6
Fragment: 1592-1601
Site: PBM
Sequence: ADENEQVSAV
Construct: Biotin-Ahx-ADENEQVSAV

Protein B

Protein: FRMPD2
Fragment: 944-1038
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.48
Normalized holdup BI: 0.53
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.85
PUBMED ID: 36115835
Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 2

Protein A

Protein: RAPGEF6
Fragment: 1592-1601
Site: PBM
Sequence: ADENEQVSAV
Construct: Biotin-Ahx-ADENEQVSAV

Protein B

Protein: FRMPD2
Fragment: 1073-1166
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Normalized holdup BI: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 3

Protein A

Protein: RAPGEF6
Fragment: 1592-1601
Site: PBM
Sequence: ADENEQVSAV
Construct: Biotin-Ahx-ADENEQVSAV

Protein B

Protein: FRMPD2
Fragment: 770-861
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0
Normalized holdup BI: -0
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.