Interaction Details


Details of experiments between Q8TEU7 and Q15700
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: RAPGEF6
Fragment: 1592-1601
Site: PBM
Sequence: ADENEQVSAV
Construct: Biotin-Ahx-ADENEQVSAV

Protein B

Protein: DLG2
Fragment: 418-513
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.07
Normalized holdup BI: 0.07
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.63
PUBMED ID: 36115835
Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 2

Protein A

Protein: RAPGEF6
Fragment: 1592-1601
Site: PBM
Sequence: ADENEQVSAV
Construct: Biotin-Ahx-ADENEQVSAV

Protein B

Protein: DLG2
Fragment: 189-283
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.06
Normalized holdup BI: 0.06
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.57
PUBMED ID: 36115835
Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.