Interaction Details


Details of experiments between Q8TDW7 and Q14005
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: FAT3
Fragment: 4548-4557
Site: PBM
Sequence: FVETQHQTQV
Construct: biotin-ttds-FVETQHQTQV

Protein B

Protein: IL16
Fragment: 1106-1201
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Normalized holdup BI: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: FAT3
Fragment: 4548-4557
Site: PBM
Sequence: FVETQHQTQV
Construct: biotin-ttds-FVETQHQTQV

Protein B

Protein: IL16
Fragment: 1203-1332
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0
Normalized holdup BI: 0
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: FAT3
Fragment: 4548-4557
Site: PBM
Sequence: FVETQHQTQV
Construct: biotin-ttds-FVETQHQTQV

Protein B

Protein: IL16
Fragment: 210-306
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.02
Normalized holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835