Interaction Details


Details of experiments between Q8TCX5 and P27037
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: RHPN1
Fragment: 504-602
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ACVR2A
Fragment: 504-513
Site: PBM
Sequence: VDFPPKESSL
Construct: biotin-ttds-VDFPPKESSL
Average holdup BI: 0.23
Normalized holdup BI: 0.23
Immobilized partner concentration (10-6M): 33
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.97
PUBMED ID: 36115835