Interaction Details


Details of experiments between Q8TCX5 and P03126
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: RHPN1
Fragment: 504-602
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Sequence: SSRTRRETQL
Construct: biotin-ttds-SSRTRRETQL
Average holdup BI: 0.57
Holdup BI standard deviation: 0.27
Immobilized partner concentration (10-6M): 15
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 6
Measured pKd: 5.01
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: RHPN1
Fragment: 504-602
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Sequence: SSRTRRETQL
Construct: Biotin-ado-ado-SSRTRRETQL
Average holdup BI: 0.39
Normalized holdup BI: 0.39
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.59
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 3

Protein A

Protein: RHPN1
Fragment: 504-602
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Sequence: SSRTRRETQL
Construct: biotin-ttds-SSRTRRETQL
Average holdup BI: 0.23
Normalized holdup BI: 0.23
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.24
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 4

Protein A

Protein: RHPN1
Fragment: 504-602
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Sequence: SSRTRRETQL
Construct: biotin-ttds-SSRTRRETQL
Average holdup BI: 0.23
Normalized holdup BI: 0.23
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.23
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: RHPN1
Fragment: 504-602
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Modification: (TPO)156
Sequence: SSRTRRETQL
Construct: Biotin-ttds-SSRTRREpTQL
Average holdup BI: -0.1
Immobilized partner concentration (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: RHPN1
Fragment: 504-602
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Modification: (TPO)156
Sequence: SSRTRRETQL
Construct: Biotin-ttds-SSRTRREpTQL
Average holdup BI: -0.07
Normalized holdup BI: -0.07
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835